| 质粒名称: | lenti sgRNA(MS2)_puro backbone |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 73795 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | plenti |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 10221 bp |
| 载体类型: | 哺乳细胞表达，Lentiviral, CRISPR |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 启动子 | U6 and EF1A |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | GAG GGC CTA TTT CCC ATG ATT CCT TCA TAT |
| 3' 测序引物 | cctagaaggtccattagctgcaaagattcc |
|
文献引用方法 |
|
| 材料和方法部分: | lenti sgRNA(MS2)_puro backbone was a gift from Feng Zhang (爱迪基因 plasmid # 73795) |
| 参考文献部分: | Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 10.1038/nature14136 PubMed 25494202 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
_puro backbone质粒图谱.png)
载体简介
载体序列
LOCUS lenti sgRNA(MS2)_puro backbone 10222 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION 3rd generation lenti sgRNA cloning backbone with MS2 loops at
tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains
BsmBI sites for insertion of spacer sequences..
ACCESSION .
VERSION .
KEYWORDS lenti sgRNA(MS2)_puro backbone
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10222)
AUTHORS Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena
C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
TITLE Genome-scale transcriptional activation by an engineered CRISPR-Cas9
complex.
JOURNAL Nature. 2014 Dec 10. doi: 10.1038/nature14136.
PUBMED 25494202
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|lenti sgRNA(MS2)_puro backbone|
FEATURES Location/Qualifiers
source 1..10222
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(81..101)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 86..415
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 266..401
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 328..347
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 463..510
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 529..903
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 1033..1154
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1070..1089)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1124..1143
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(1203..1219)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1203..1219)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1216..1238)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1227..1243
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1251..1281)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1296..1317
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1434..1451)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1605..2193)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1685..1704)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2364..3224)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2987..3006
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3225..3329)
/gene="bla"
/label=AmpR promoter
primer_bind complement(3404..3423)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 3595..3974
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3976..4174
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4129..4149
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 4192..4372
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 4419..4544
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5037..5270
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 5797..5914
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 5964..6204
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 5964..5984
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 6135..6154
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 6250..6268
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 6320..6338
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
promoter 6462..7640
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 6693..7631
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 7588..7608
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 7647..8246
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(7647..7666)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 8143..8163
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 8271..8859
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8324..8344)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8742..8753)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(8862..8878)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8863..8879)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 9384..9564
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(9590..9607)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9596..9820
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9866..72
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9953..9972)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 10163..10184
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
61 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
121 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
181 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
241 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
301 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
361 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
421 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
481 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
541 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
601 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
661 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
721 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
781 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
841 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
901 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
961 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
1021 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
1081 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
1141 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
1201 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
1261 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
1321 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
1381 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
1441 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
1501 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
1561 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
1621 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
1681 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
1741 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
1801 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
1861 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
1921 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
1981 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
2041 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
2101 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
2161 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
2221 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
2281 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
2341 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
2401 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
2461 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
2521 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
2581 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
2641 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
2701 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
2761 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
2821 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
2881 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
2941 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
3001 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
3061 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
3121 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
3181 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
3241 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
3301 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
3361 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
3421 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
3481 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
3541 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
3601 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
3661 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
3721 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
3781 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
3841 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
3901 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
3961 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
4021 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
4081 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
4141 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
4201 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
4261 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
4321 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
4381 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
4441 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
4501 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
4561 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
4621 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
4681 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
4741 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
4801 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
4861 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
4921 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
4981 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
5041 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
5101 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
5161 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
5221 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
5281 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
5341 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
5401 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
5461 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
5521 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
5581 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
5641 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
5701 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
5761 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
5821 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
5881 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
5941 tccagtttgg ttaattagct agcgagggcc tatttcccat gattccttca tatttgcata
6001 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
6061 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
6121 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
6181 ctttatatat cttgtggaaa ggacgaaaca ccggagacgg gataccgtct ctgttttaga
6241 gctaggccaa catgaggatc acccatgtct gcagggccta gcaagttaaa ataaggctag
6301 tccgttatca acttggccaa catgaggatc acccatgtct gcagggccaa gtggcaccga
6361 gtcggtgctt tttttggatc ctgcaaagat ggataaagtt ttaaacagag aggaatcttt
6421 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
6481 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
6541 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
6601 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
6661 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
6721 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
6781 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
6841 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
6901 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
6961 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
7021 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
7081 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
7141 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
7201 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
7261 ggccgcttcc cggccctgct gcagggagct caaaatgaag gacgcggcgc tcgggagagc
7321 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
7381 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
7441 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
7501 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
7561 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
7621 ttccatttca ggtgtcgtga tgtacaatga ccgagtacaa gcccacggtg cgcctcgcca
7681 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
7741 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
7801 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
7861 ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
7921 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
7981 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc
8041 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
8101 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
8161 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
8221 gcatgacccg caagcccggt gcctgagaat tcgatatcaa gcttatcggt aatcaacctc
8281 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
8341 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
8401 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
8461 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
8521 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
8581 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
8641 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
8701 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
8761 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
8821 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
8881 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
8941 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
9001 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
9061 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
9121 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
9181 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
9241 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
9301 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
9361 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
9421 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
9481 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
9541 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
9601 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
9661 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
9721 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
9781 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
9841 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
9901 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
9961 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
10021 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
10081 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
10141 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
10201 atctcggtct attcttttga tt
//
